Coming soon: FL!Ps™
FL!Ps are molecular probes developed specifically for polarization microscopy. The probes allow easy, quantitative imaging of many molecular processes of cell signaling (activation/inactivation of GPCRs, G proteins, GTPases, receptor tyrosine kinases,...)
Coming soon: Equaliz=r™
Our Equaliz=r™ (patent pending) modulates laser power during fast laser scanning by resonant galvo scanners to compensate for the changing scanning speed, resulting in even illumination power throughout the whole scanning cycle!
1P FL!Pper™
Our rapid polarization modulator rotates the excitation beam polarization between acquisition of individual lines (or even pixels) by a laser scanning confocal microscope. This way, each pixel is imaged twice, using two different polarizations, within one image. Because information with both polarizations is acquired virtually simultaneously, there are no problems caused by cell movement, and even rapid molecular events can be reliably observed and monitored. The 1P FL!Pper is compatible with the full range of visible excitation wavelengths (405-640 nm). In combination with our accompanying Polaris+ software, the 1P FL!Pper it allows easy, accurate quantification of linear dichroism. To learn more about the 1P FL!Pper, including pricing information, contact our sales team.
Coming soon: Equaliz=r™
Our Equaliz=r™ (patent pending) modulates laser power during fast laser scanning by resonant galvo scanners to compensate for the changing scanning speed, resulting in even illumination power throughout the whole scanning cycle!
1P FL!Pper™
Our rapid polarization modulator rotates the excitation beam polarization between acquisition of individual lines (or even pixels) by a laser scanning confocal microscope. This way, each pixel is imaged twice, using two different polarizations, within one image. Because information with both polarizations is acquired virtually simultaneously, there are no problems caused by cell movement, and even rapid molecular events can be reliably observed and monitored. The 1P FL!Pper is compatible with the full range of visible excitation wavelengths (405-640 nm). In combination with our accompanying Polaris+ software, the 1P FL!Pper it allows easy, accurate quantification of linear dichroism. To learn more about the 1P FL!Pper, including pricing information, contact our sales team.
2P FL!Pper™
Similar to our 1P FL!PPer, but tailored for two-photon excitation laser wavelengths (700-1200 nm). Unlike single-photon excitation, two-photon excitation allows not just observations of linear dichroism, but accurate determinations of orientations of fluorescent molecules with respect to membranes or filaments. The 2P FL!Pper is compatible with virtually any existing two-photon microscope. The accompanying Polaris+ software allows not only processing of acquired images and display of linear dichroism as color hues on various scales and in various color combinations, but also quantitative measurements of linear dichroism in living cells and determinations of molecular orientations. To learn more about the 2P FL!Pper, including pricing information, contact our sales team.
Similar to our 1P FL!PPer, but tailored for two-photon excitation laser wavelengths (700-1200 nm). Unlike single-photon excitation, two-photon excitation allows not just observations of linear dichroism, but accurate determinations of orientations of fluorescent molecules with respect to membranes or filaments. The 2P FL!Pper is compatible with virtually any existing two-photon microscope. The accompanying Polaris+ software allows not only processing of acquired images and display of linear dichroism as color hues on various scales and in various color combinations, but also quantitative measurements of linear dichroism in living cells and determinations of molecular orientations. To learn more about the 2P FL!Pper, including pricing information, contact our sales team.
Polaris/Polaris+: software for polarization-resolved fluorescence microscopy
Our polarization modulators come with software that allows facile display and quantitation of linear dichroism: Polaris (for analyzing any pair of images acquired with distinct excitation light polarizations) and Polaris+ (for analyzing images acquired with our 1P FL!Pper or 2P FL!Pper polarization modulators). To learn more about the Polaris/Polaris+, including pricing information, contact our sales team.
Both versions provide sophisticated, yet easy to use tools that will allow even subtle linear dichroism to be reliably observed and measured. Our software builds on years of expertise in polarization microscopy, and will provide you with both published and proprietary scientifically correct algorithms for image processing and analysis. Both versions of our software allow a wide range of choices for visualizing linear dichroism, including choices suitable for people with color vision impairments. Polaris+ has been designed to streamline processing of time series and z-stacks. Polaris+ takes advantage of our detailed knowledge of all parts of a polarization microscope to deliver results that cannot be matched.
Thanks to our software, you can have a complete polarization microscopy system, capable of delivering publication-quality results, right after installation.
Thanks to our software, you can have a complete polarization microscopy system, capable of delivering publication-quality results, right after installation.