1P FL!Pper™
Our rapid polarization modulator rotates the excitation beam polarization between acquisition of individual lines (or even pixels) by a laser scanning confocal microscope. This way, each pixel is imaged twice, using two different polarizations, within one image. Because information with both polarizations is acquired virtually simultaneously, there are no problems caused by cell movement, and even rapid molecular events can be reliably observed and monitored. The 1P FL!Pper is compatible with the full range of visible excitation wavelengths (405-640 nm). In combination with our accompanying Polaris+ software, the 1P FL!Pper it allows easy, accurate quantification of linear dichroism. To learn more about the 1P FL!Pper, including pricing information, contact our sales team.
2P FL!Pper™
Similar to our 1P FL!PPer, but tailored for two-photon excitation laser wavelengths (700-1200 nm). Unlike single-photon excitation, two-photon excitation allows not just observations of linear dichroism, but accurate determinations of orientations of fluorescent molecules with respect to membranes or filaments. The 2P FL!Pper is compatible with virtually any existing two-photon microscope. The accompanying Polaris+ software allows not only processing of acquired images and display of linear dichroism as color hues on various scales and in various color combinations, but also quantitative measurements of linear dichroism in living cells and determinations of molecular orientations. To learn more about the 2P FL!Pper, including pricing information, contact our sales team.
